Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cell Biology
Biomolecules
Biomolecules
Tumor Necrosis Factor-α human
Tumor Necrosis Factor-α human from MilliporeSigma
Be the first to write a review!
Citations:
(60)
Supplier Page
Supplier Page from
MilliporeSigma for
Tumor Necrosis Factor-α human
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Tumor Necrosis Factor-α human
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
H8916
Molecular Weight
17 kDa (glycosylated) ~17.4 kDa
Species
human
Molecule Name
ADRA1D, ADRA1A, ADRA1B
NCBI Full Gene Name
adrenoceptor alpha 1A, adrenoceptor alpha 1B, adrenoceptor alpha 1D
NCBI Gene Aliases
ADRA1A, ADRA1C, ALPHA1, ADRA1, ALPHA1BAR, ADRA1R, DAR, ADRA1L1, dJ779E11.2, ALPHA1AAR
Applications
mammalian cell culture
Quantity
10µG
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (60)
(1)
Differential cardiomyocyte transcriptomic remodeling during in vitro Trypanosoma cruzi infection using laboratory strains provides implications on pathogenic host responses
Trop Med Health
December 7, 2023
Katherine-Sofia Candray-Medina, Yu Nakagama, Masamichi Ito, Shun Nakagama, Evariste Tshibangu-Kabamba, Norihiko Takeda, Yuki Sugiura, Yuko Nitahara, Yu Michimuko-Nagahara, Natsuko Kaku,
et al.
was collected to infect HL-1
cardiomyocytes
at an MOI of 10:1.
Mouse
TNF-α (100 ng/mL;
Sigma-Aldrich
H8916
, Missouri, USA) was added to the culture medium of the positive control wells [19, 21, 26–31]. CCdT
(2)
Rheumatoid Synovial Fluid and Acidic Extracellular pH Modulate the Immunomodulatory Activity of Urine-Derived Stem Cells
International Journal of Molecular Sciences
November 1, 2023
Michaela Cehakova, Dana Ivanisova, Magdalena Strecanska, Jana Plava, Zuzana Varchulova Novakova, Andreas Nicodemou, Stefan Harsanyi, Martina Culenova, Sona Bernatova, Lubos Danisovic
in SFM hg DMEM (Sigma-Aldrich, St. Louis, MO, USA) with RASFs (1 mg/mL) and recombinant human TNF-α (
H8916
,
Sigma-Aldrich
, St. Louis, MO, USA; 10 ng/mL) for 48 h. 4.6. ATSCs
Treatment Protocol
To evaluate
(3)
Inhibition of TGF-β signaling, invasion, and growth of cutaneous squamous cell carcinoma by PLX8394
Oncogene
October 20, 2023
Elina Siljamäki, Pilvi Riihilä, Ujjwal Suwal, Liisa Nissinen, Pekka Rappu, Markku Kallajoki, Veli-Matti Kähäri, Jyrki Heino
or as a control with 0.1% DMSO o/n at 37 °C. Next day, the cells were treated with TNF-α (10 ng/ml;
H8916
,
Sigma-Aldrich
) and IL-1β (10 ng/ml; 407615-5, VWR) for 30 min/+37 °C, or with D-sorbitol (400 mM, Sigma
(4)
Effect of P53 nuclear localization mediated by G3BP1 on ferroptosis in acute liver failure
Apoptosis : an international journal on programmed cell death
August 1, 2023
Li W, Li W, Li X, Wang L, Wang Y.
No. 12,483,020) were purchased from Gibco (Grand Island, USA). LPS (Cat. No.
L4391
), TNF-α (Cat. No.
H8916
) and D-galactosamine (D-Gal) (Cat. No. G0500) were purchased from
Sigma
(Grand Island, USA). A bicinchoninic
(5)
Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells
iScience
July 21, 2023
Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen,
et al.
96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (
Sigma Aldrich
,
H8916
, 100ng.mL-1) or 0.1%
Bovine Serum Albumin
(
Sigma Aldrich
, A0281-10G) in
Astrocyte medium
(ScienCell,
(6)
Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells
iScience
July 21, 2023
Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick
LB Broth (Lennox
),
Sigma Aldrich
(UK),Cat#
L3022
Tumor Necrosis Factor-α human
,
Sigma Aldrich
(UK),Cat#
H8916
Recombinant Human VEGF 165 Protein
(with carrier),
R&D systems
(USA),Cat#
293-VE-050
Histamine dihydrochloride
,
Sigma Aldrich
(7)
The Impact of Hydroxychloroquine on Primary Feto-Placental Endothelial Cells from Healthy and Early-Onset Preeclamptic Placentas
International Journal of Molecular Sciences
June 30, 2023
Maja Gajić, Bianca Schröder-Heurich, Monika Horvat Mercnik, Mila Cervar-Zivkovic, Christian Wadsack, Frauke von Versen-Höynck, Karoline Mayer-Pickel
sterile water (Fresenius Kabi, Bad Homburg, Germany) to the concentration of 10 mg/mL. Cytokines: TNF-α (
H8916
,
Merck
, Darmstadt, Germany), IL-6 (
I1395
,
Merck
, Darmstadt, Germany) and
recombinant human IL-1β
(200-Q1B,
(8)
TMEM123 a key player in immune surveillance of colorectal cancer
Frontiers in Immunology
June 23, 2023
Elisa Pesce, Chiara Cordiglieri, Mauro Bombaci, Serenella Eppenberger-Castori, Stefania Oliveto, Cristina Manara, Mariacristina Crosti, Caner Ercan, Mairene Coto, Andrea Gobbini,
et al.
P0409
Rapamycin,
Sigma
-
Merk
,Cat#
553210
RIPA buffer
,Sigma-
Merk
,Cat#
R0278
tumor
necrosis
factor alpha,Sigma-
Merk
,Cat#
H8916
WheatGermAgglutinine- OregonGreen 488,Invitrogen Molecular Probes -
Thermo Fisher
,Cat#
W6748
Critical
(9)
Postmortem Brains from Subjects with Diabetes Mellitus Display Reduced GLUT4 Expression and Soma Area in Hippocampal Neurons: Potential Involvement of Inflammation
Cells
April 25, 2023
Caio Yogi Yonamine, Marisa Passarelli, Claudia Kimie Suemoto, Carlos Augusto Pasqualucci, Wilson Jacob-Filho, Venâncio Avancini Ferreira Alves, Suely Kazue Nagahashi Marie, Maria Lucia Correa-Giannella, Luiz Roberto Britto, Ubiratan Fabres Machado
subjected to four different
experimental design
protocols: (1) tumor necrosis factor (TNF) (
Sigma-Aldrich
, #
H8916
) at 20 ng/mL for 3 h; (2) glycated albumin (GA) or non-glycated albumin at 0.4 mg/mL for 24 h; (3) RGFP-996
(10)
Expression of GnT-III decreases chemoresistance via negatively regulating P-glycoprotein expression: Involvement of the TNFR2-NF-κB signaling pathway
Journal of Biological Chemistry
April 1, 2023
Wanli Song, Caixia Liang, Yuhan Sun, Sayaka Morii, Shin Yomogida, Tomoya Isaji, Tomohiko Fukuda, Qinglei Hang, Akiyoshi Hara, Miyako Nakano,
et al.
from Vector Laboratories; E4-PHA agarose (J311), which is from J-OIL MILLS; Recombinant human TNFα (
H8916
),
BAY 11-7082
(
B5556
), dasatinib (SML2589), and doxycycline hyclate (D9891), which were acquired from
(11)
A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral Infections
Advanced Science
March 1, 2023
Patrick Carius, Annemarie Jungmann, Marco Bechtel, Alexander Grißmer, Annette Boese, Gilles Gasparoni, Abdulrahman Salhab, Ralf Seipelt, Klaus Urbschat, Clémentine Richter,
et al.
stimulation medium supplemented with tumor necrosis factor alpha (TNF α) (
human
25 ng mL−1;
Sigma‐Aldrich
,
H8916
‐10UG) and interferon gamma (INF γ) (
human
30 ng mL−1;
Sigma‐Aldrich
,
I17001
‐100UG). The stimulation
(12)
New Insights into Bitopic Orthosteric/Allosteric Ligands of Cannabinoid Receptor Type 2
International Journal of Molecular Sciences
January 21, 2023
Rebecca Ferrisi, Beatrice Polini, Caterina Ricardi, Francesca Gado, Kawthar A. Mohamed, Giovanna Baron, Salvatore Faiella, Giulio Poli, Simona Rapposelli, Giuseppe Saccomanni,
et al.
penicillin (100 U/mL) (
Sigma-Aldrich
, Milan, Italy). LPS (Cat. number
L4391
) and TNFα (Cat. number
H8916
) were purchased from
Sigma-Aldrich
(Milan, Italy). 3.2. HitHunter cAMP
Assay
This method was conducted
(13)
Inhibition of NF-kB/IL-6/JAK2/STAT3 Pathway and Epithelial-Mesenchymal Transition in Breast Cancer Cells by Azilsartan
Molecules
November 13, 2022
Rania Alaaeldin, Fares E. M. Ali, Amany Abdlrehim Bekhit, Qing-Li Zhao, Moustafa Fathy
targeting and inhibiting the activated NF-kB pathway in MCF-7 and MDA-MB-231
breast cancer
cells, TNFα (#
H8916
,
Sigma Aldrich
, St Louis, MO, USA) was used to induce NF-kB in both MCF-7 and MDA-MB-231
cancer
cells,
(14)
The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis
BMC Immunology
September 10, 2022
Xue Bai, Peng-Cheng Xu, Tong Chen, Hao-Miao Zhang, Si-Jing Wu, Xia Yang, Shan Gao, Jun-Ya Jia, Jian-Qing Jiang, Tie-Kun Yan
S100A12
by
neutrophils
stimulated with ANCA
Neutrophils
(1 × 106/ml) were primed with 2 ng/ml TNF-α (
H8916
,
Sigma-Aldrich
) at 37 °C for 15 min and cultured with normal control IgG from healthy donors and different
(15)
Regulation of Adipose Progenitor Cell Expansion in a Novel Micro-Physiological Model of Human Adipose Tissue Mimicking Fibrotic and Pro-Inflammatory Microenvironments
Cells
September 7, 2022
Dani V, Bruni-Favier S, Chignon-Sicard B, Loubat A, Doglio A, Dani C.
Germany) supplemented with 2% FCS, 22.5 μg/mL ascorbic acid, 1 μg/mL heparin and 10 ng/mL TNFα (Cat:
H8916
,
Sigma-Aldrich
, St. Louis, MO, USA) or 5 ng/mL
TGFβ1
(Cat: 100-21, Preprotech, Neuilly-sur-Seine, France). 2.5.
(16)
Culture-Associated DNA Methylation Changes Impact on Cellular Function of Human Intestinal Organoids
Cellular and Molecular Gastroenterology and Hepatology
August 28, 2022
Rachel D. Edgar, Francesca Perrone, April R. Foster, Felicity Payne, Sophia Lewis, Komal M. Nayak, Judith Kraiczy, Aurélie Cenier, Franco Torrente, Camilla Salvestrini,
et al.
splitting in the growth medium, followed by 24 hours of treatment with recombinant
human
protein TNFα (
H8916
;
Sigma Aldrich
, Burlington, MA) at 40 ng/mL or
IFNγ
(
PHC4031
;
Life Technologies
, Carlsbad, CA) at 20
(17)
Quercetin, a flavonoid, combats rotavirus infection by deactivating rotavirus-induced pro-survival NF-κB pathway
Frontiers in Microbiology
August 2, 2022
Shreya Banerjee, Rakesh Sarkar, Arpita Mukherjee, Shin-ichi Miyoshi, Kei Kitahara, Prolay Halder, Hemanta Koley, Mamta Chawla-Sarkar
USA. Protease inhibitor cocktail (P2714),
phosphatase inhibitor cocktail 2
(
P5726
),
MTT
(
M5655
), TNFα (
H8916
), and Bay11-7082 (
B5556
) were procured from
Merck
(Sigma-Aldrich, USA). Rabbit polyclonal antibody against
(18)
Pro-inflammatory cytokines mediate the epithelial-to-mesenchymal-like transition of pediatric posterior fossa ependymoma
Nature communications
July 8, 2022
Aubin RG, Troisi EC, Montelongo J, Alghalith AN, Nasrallah MP, Santi M, Camara PG.
treated with 4 ng/ml TGF-β1 (
Sigma-Aldrich
, cat. #
H8541
-5UG), 10 ng/ml TNF-α (
Sigma-Aldrich
, cat. #
H8916
-10UG), or both, for 5 days before RNA extraction. Media was replenished every 48 h. Cells were plated
(19)
Characterization and Validation of In Vitro and In Vivo Models to Investigate TNF-α-Induced Inflammation in Retinal Diseases
Translational Vision Science & Technology
May 17, 2022
Carina M. Weigelt, Nina Zippel, Holger Fuchs, Anna-Kaisa Rimpelä, Tanja Schönberger, Birgit Stierstorfer, Remko A. Bakker, Norbert H. Redemann
TNF-α–induced
expression analysis
assays
, cells were stimulated with 10 ng/mL recombinant human TNF-α (
H8916
,
Sigma Aldrich Corp
.) for 24 hours with or without 58.8 nM golimumab (Simponi, MSD Sharp & Dohme). THP-1
(20)
Metformin Treatment Regulates the Expression of Molecules Involved in Adiponectin and Insulin Signaling Pathways in Endometria from Women with Obesity-Associated Insulin Resistance and PCOS
International Journal of Molecular Sciences
April 1, 2022
Maria Lorena Oróstica, Isis Astorga, Francisca Plaza-Parrochia, Cristian Poblete, Rodrigo Carvajal, Víctor García, Carmen Romero, Margarita Vega
(Merck), Darmstadt, Germany); a
human
recombinant protein called TNFα (
tumor
necrosis
factor alpha, #
H8916
,
Sigma-Aldrich
, MO, USA) and metformin (
1,1-Dimethylbiguanide hydrochloride
, #1115-70.4,
Sigma-Aldrich
Page
<<
<
1
2
3
>
>>
Reviews
Be the First to Write a Review!
Tumor Necrosis Factor-α human from MilliporeSigma
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top